摘要 : Mitigating sepsis-induced severe organ dysfunction with magneticnanoparticles has shown remarkable advances in extracorporeal bloodtreatment. Nevertheless, treating large septic animals remains challengingdue to insufficient magne... 展开 Mitigating sepsis-induced severe organ dysfunction with magneticnanoparticles has shown remarkable advances in extracorporeal bloodtreatment. Nevertheless, treating large septic animals remains challengingdue to insufficient magnetic separation at rapid blood flow rates (>6 L h−1)and limited incubation time in an extracorporeal circuit. Herein,superparamagnetic nanoclusters (SPNCs) coated with red blood cell (RBC)membranes are developed, which promptly capture and magnetically separatea wide range of pathogens at high blood flow rates in a swine sepsis model.The SPNCs exhibited an ultranarrow size distribution of clustered iron oxidenanocrystals and exceptionally high saturation magnetization (≈ 90 emu g~（−1）)close to that of bulk magnetite. It is also revealed that CD47 on the RBCsallows the RBC-SPNCs to remain at a consistent concentration in the blood byevading innate immunity. The uniform size distribution of the RBC-SPNCsgreatly enhances their effectiveness in eradicating various pathogenicmaterials in extracorporeal blood. The use of RBC-SPNCs for extracorporealtreatment of swine infected with multidrug-resistant E. coli is validated andfound that severe bacteremic sepsis-induced organ dysfunction issignificantly mitigated after 12 h. The findings highlight the potentialapplication of RBC-SPNCs for extracorporeal therapy of severe sepsis in largeanimal models and potentially humans. 收起