摘要 : Plate-based single-cell glycan and RNA sequencing (scGR-seq) is previouslydeveloped to realize the integrated analysis of glycome and transcriptome insingle cells. However, the sample size is limited to only a few hundred cells.He... 展开 Plate-based single-cell glycan and RNA sequencing (scGR-seq) is previouslydeveloped to realize the integrated analysis of glycome and transcriptome insingle cells. However, the sample size is limited to only a few hundred cells.Here, a droplet-based scGR-seq is developed to address this issue byadopting a 10x Chromium platform to simultaneously profile ten thousandcells’ glycome and transcriptome in single cells. To establish droplet-basedscGR-seq, a comparative analysis of two distinct cell lines is performed:pancreatic ductal adenocarcinoma cells and normal pancreatic duct cells.Droplet-based scGR-seq revealed distinct glycan profiles between the two celllines that showed a strong correlation with the results obtained by flowcytometry. Next, droplet-based scGR-seq is applied to a more complexsample: peripheral blood mononuclear cells (PBMC) containing variousimmune cells. The method can systematically map the glycan signature foreach immune cell in PBMC as well as glycan alterations by cell lineage.Prediction of the association between the glycan expression and the geneexpression using regression analysis ultimately leads to the identification of aglycan epitope that impacts cellular functions. In conclusion, thedroplet-based scGR-seq realizes the high-throughput profiling of the distinctcellular glyco-states in single cells. 收起