摘要 : The long start-up time and facile biomass loss of aerobic granular sludge (AGS) impede its application for actual wastewater treatment. The present study investigates a novel assist-aggregation strategy based on Aspergillus tubing... 展开 The long start-up time and facile biomass loss of aerobic granular sludge (AGS) impede its application for actual wastewater treatment. The present study investigates a novel assist-aggregation strategy based on Aspergillus tubingensis (AT) mycelium pellets to accelerate sludge granulation, and engineered Fe_3O_4 nanoparticles (NPs) were used to further enhance flocculent sludge (FS) aggregation. The AT mycelium pellets, modified by 0.5 g/L Fe_3O_4@SiO_2-QC NPs (AT-V), had a more compact internal structure than the unmodified group (AT-I). The content of extracellular polymeric substances (EPS) and the zeta potential values were observed to increase from 39.86 mg/gVSS and -9.19 mv for AT-I to 69.64 mg/gVSS and 235 mv for AT-V, respectively. In optimized cultivation conditions, the aggregated sludge biomass of AT-V reached 1.54 g/g. An original AT-based AGS (AT-AGS) with a high biological activity (64.45 mgO_2/gVSSh as specific oxygen uptake rate) and enhanced velocity (58.22 m/h) was developed in only 9 days. The removal efficiencies of total nitrogen (TN) and total phosphorus (TP) of the AT-AGS were 12.24% and 16.29% higher than those of the inoculated FS under high feeding load. Additionally, the analysis of cyclic diguanylate (c-di-GMP) and con-focal microscope images implied that polysaccharide (PS) of EPS played an important role in maintaining the stability of the AT-AGS. Finally, the dominant functional species contributing to sludge aggregation and pollutants removal of the AT-AGS showed a larger richness and diversity than those of the inoculated FS. This study might provide a novel high-efficiency strategy for the fast formation of AGS. 收起