摘要 : Protoplasts were isolated from needles of in vitro shoots initiated from short shoot buds of stecklings from an 8-year old hybrid larch (Larix x eurolepis Henry) tree. Protoplast yield and viability have reached, respectively 8.8 ... 展开 Protoplasts were isolated from needles of in vitro shoots initiated from short shoot buds of stecklings from an 8-year old hybrid larch (Larix x eurolepis Henry) tree. Protoplast yield and viability have reached, respectively 8.8 x 10(5) protoplasts/g fresh weight and 91.8% when needles were enzymatically digested for 16 h in osmoticum containing 0.5% Cellulase R-10, 0.5% Macerozyme R-10 and 0.5% BSA. First cell divisions were observed after 2 days of culture in a modified liquid (or solid) Murashige and Skoog medium (MSG) containing 2 mg/l 2,4-D and 0.5 mg/l BAP. After 1 week, 15-25% of the protoplasts had divided, and some had formed microcolonies with a plating efficiency from 3.9-7.9%. Protoplast-derived cells which had undergone division were concentrated mainly in size classes between 25.0 and 44.5 mu m, and division frequency increased with diameter. [References: 29] 收起