摘要 : ? 2022 Elsevier B.V.The trematode Dicrocoelium lanceatum known as lancet fluke, is a causative agent of dicrocoeliosis, a widespread parasitic disease of the grazing ruminants. The investigation of the major neurotransmitters and ... 展开 ? 2022 Elsevier B.V.The trematode Dicrocoelium lanceatum known as lancet fluke, is a causative agent of dicrocoeliosis, a widespread parasitic disease of the grazing ruminants. The investigation of the major neurotransmitters and their functions are an important step in the development of a new pharmacological strategy of the struggle against the dicrocoeliosis affecting the neuronal signal substances and the functions of its nervous system. The aim of this work was to study the presence and localization of the neurotransmitter serotonin (5-HT, 5-Hydroxytryptamine) in the nervous system of D. lanceatum using immunocytochemical technique and confocal laser scanning microscopy. For the first time the data on the presence and distribution of serotonin-immunopositive components in the central and peripheral compartments of the nervous system of D. lanceatum has been obtained. Serotonin-immunopositive neurons and neurites were identified in paired brain ganglia, in the brain commissure, longitudinal nerve cords and connective nerve commissures. The innervation of the oral and ventral suckers by serotonergic nerve structures was revealed. The distal part of the reproductive system and the region of the reproductive pore were intensively innervated by serotonergic neurites. Serotonin-immunopositive neurons and neurites were also revealed in the proximal region of the reproductive system. The data obtained suggest that the serotonergic nervous system is involved in the regulation of the attachment organs and the reproductive system functions in D. lanceatum. The new results on the morphological and functional organization of the D. lanceatum nervous system increase our knowledge of the structure and function of nervous system of trematodes of various taxonomic groups and support the possibility of the exploitation of the serotonergic system of the parasite as a target for anthelmintic drugs. 收起