摘要 : The selected thermotolerant,lactose-utilizing yeast strain Kluyveromyces marxianus NBIMCC 8362 possesses high specific D-amino acid oxidase activity(60 Ug~(-1)),which was increased nine-fold(545 Ug~(-1))by design of the growth med... 展开 The selected thermotolerant,lactose-utilizing yeast strain Kluyveromyces marxianus NBIMCC 8362 possesses high specific D-amino acid oxidase activity(60 Ug~(-1)),which was increased nine-fold(545 Ug~(-1))by design of the growth medium and conditions for D-amino oxidase induction.Applying an optimized simple and rapid procedure for chemical permeabilization of K.marxianus cells with the cationic detergent cetyltrimethylammonium bromide,the enzyme activities(D-amino acid oxidase and catalase)of the cells have been further increased for up to 43-and 58-fold,respectively.However,the enzyme activities of the permeabilized cells decreased rapidly due to the leakage of the enzymes.Treating the permeabilized cells with 0.1% glutaraldehyde at 4°C for 10 min stabilized the enzyme in the cells and prevented their outflow.The process is stable for 10 cycles and the productivity measured was 16.6 mmmol l~(-1)h~(-1).The D-alanine transformation efficiency of K.marxianus permeabihzed and GA entrapted cells was 98%. 收起