摘要 : BACKGROUND: Immature oocyte cryopreservation is a therapeutic option in assisted reproductive technology. OBJECTIVE: To evaluate the effects of supplementing oocyte maturation medium with human follicular fluid (hFF), zinc and cop... 展开 BACKGROUND: Immature oocyte cryopreservation is a therapeutic option in assisted reproductive technology. OBJECTIVE: To evaluate the effects of supplementing oocyte maturation medium with human follicular fluid (hFF), zinc and copper. MATERIALS AND METHODS: Four different maturation media supplemented with 10% follicular fluid, 4 mu g/mL copper and 1 mu g/mL zinc were used for vitrified-warmed oocytes of mouse. RESULTS: Maturation rate was the highest (63.3%) in the presence of zinc. Cleavage and blastocyst rates in groups with copper and zinc were significantly higher than the control group (39.9% and 46.4% vs. 28.8%), without any significant difference between Zn and Cu. CONCLUSION: Our findings indicate the high importance of using hFF as a natural medium, and also zinc and copper as two efficient trace elements in the maturation medium for vitrified-warmed oocytes. 收起