摘要 : Estrogen has an important role in the reconstruction of a new vascular network in the endometrium during each menstrual cycle, however, the underlying mechanisms are incompletely understood Angiopoietin-1 (Ang-1) promotes vessel a... 展开 Estrogen has an important role in the reconstruction of a new vascular network in the endometrium during each menstrual cycle, however, the underlying mechanisms are incompletely understood Angiopoietin-1 (Ang-1) promotes vessel assembly, whereas Ang-2 and thrombospondin-1 (TSP-1) cause vessel breakdown To determine the potential effect of estrogen on the expression of these angioregulatory factors in the endometrium, Ang-1, Ang-2, TSP-1, and Tie-2 receptor mRNA levels were assessed by real-time reverse transcriptase polymerase chain reaction in glandular epithelial and stromal cells isolated from the endometrium of ovariectomized baboons treated acutely with estradiol Corresponding protein expression was assessed by immunocytochemistry and the proximity ligation assay (PLA) during advancing stages of the baboon menstrual cycle Serum estradiol levels in ovariectomized baboons were 400 pg/ml within 4-6 hr of estradiol treatment Ang-1 mRNA levels in glandular epithelial cells increased threefold (P<0 01) within 4 hr of estradiol administration In contrast, TSP-1 mRNA levels decreased four- to fivefold (P < 0 01) in endometnal glandular epithelial and stromal cells 4-6 hr after estradiol, whereas Ang-2 and Tie-2 expression was unaltered Immunostaining for Ang-1 increased, TSP-1 decreased, and Ang-2 and Tie-2 were unaltered in the endometrium during the secretory compared with the proliferative phase of the cycle Endometrial Ang-1 protein expression, quantified by PLA, increased 10-fold (P < 0 05) between the early proliferative and late proliferative/mid-secretory phases of the menstrual cycle in association with the rise in estrogen In summary, estrogen induced a rapid, divergent, and cell-specific change in expression of angiostimulatory and angioinhibitory growth factors in the endometrium of the nonhuman primate 收起