摘要 : The functional parameters of mitochondria (intactness, oxidation rates of succinate and NAD center dot H, respiratory control coefficient, ADP/O ratio, and protein content) were studied in freshly harvested and cold-stored tubers ... 展开 The functional parameters of mitochondria (intactness, oxidation rates of succinate and NAD center dot H, respiratory control coefficient, ADP/O ratio, and protein content) were studied in freshly harvested and cold-stored tubers of potato Solanum tuberosum L. cv. Skarb, transformed with a gene gox encoding the glucose oxidase from Penicillium funiculosum. Using untransformed potatoes and potatoes transformed with a vector without the target gene and vector constructs carrying the native and modified gox gene, it was shown that its expression causes changes in the rates of oxidation of the studied substrates and the degree of coupling of oxidation and phosphorylation, reduces the potential activity of the alternative oxidase, and induces the synthesis of a number of protective mitochondrial proteins involved in the regulation of the formation of ROS during storage in the cold. The role of hydrogen peroxide in the mechanisms of growth and stress resistance of plants, and its participation in the respiratory metabolism of plant cells is discussed. 收起