摘要 : Plant regeneration from callus of S. phlebopetala, an Australian native species of horticultural significance, is reported. The effects of coconut milk, kinetin, 2,4-D and BAP [benzyladenine] on callus and shoot induction were inv... 展开 Plant regeneration from callus of S. phlebopetala, an Australian native species of horticultural significance, is reported. The effects of coconut milk, kinetin, 2,4-D and BAP [benzyladenine] on callus and shoot induction were investigated. Coconutmilk promoted callus induction. Kinetin was not required for callus induction and 2,4-D induced a poor quality callus. BAP had significant effects on callus induction, growth and appearance. BAP also induced shoot initiation from callus produced from root and leaf explants. Shoot regeneration was achieved on a medium containing 5.0% coconut milk, 60 g sucrose/litre and 3 mg BAP/litre. Shoot growth required a hormone-free medium containing 20 g sucrose/litre. Regenerated shoots were rooted on medium supplemented with 4 mg IBA/litre. Ninety-six percent of the regenerated plants survived when transferred to soil under glasshouse conditions. 收起