摘要 : A lipid-coated catalytic antibody was prepared by mixing aqueous solutions of antibody and synthetic glycolipids. The lipid-coated catalytic antibody is soluble in organic solvents and showed a remarkable reactivity for hydrolysis... 展开 A lipid-coated catalytic antibody was prepared by mixing aqueous solutions of antibody and synthetic glycolipids. The lipid-coated catalytic antibody is soluble in organic solvents and showed a remarkable reactivity for hydrolysis of lipophilic esters in a buffer solution containing 20-80% DMSO (dimethyl sulfoxide). DMSO was added to solubilize the lipophilic esters and a native antibody was denatured in the same condition. Michaelis-Menten kinetics showed that the reduced reactivity of a native antibody in DMSO-buffer solutions is due to the largely deduced k(cat) value but not the small change of K-m, value. [References: 40] 收起