摘要 : Chromosome preparations of Haliotis discuss hannai larvae were subjectedto karyotype analysis using a scanning electron microscope (SEM), bandinganalysis using a salt solution treatment, and nucleolus organizer region(NOR) analysi... 展开 Chromosome preparations of Haliotis discuss hannai larvae were subjectedto karyotype analysis using a scanning electron microscope (SEM), bandinganalysis using a salt solution treatment, and nucleolus organizer region(NOR) analysis by silver staining. Standard values of the relative lengthand arm ratio of each chromosome pair in this species were determined bySEM measurements of the chromosome arm length. The arm ratios indicatedthat this species possessed 11 pairs of metacentric and seven pairs ofsubmetacentric chromosomes (2 n = 36). This is the first study ofHaliotidae in which banding is attempted (i.e., not partial banding, suchas C-banding) as well as NOR analyses. The characteristic banding patternserved as a useful marker for the identification of homologous chromosomesthat were difficult to identify using only morphological analysis. TheNORs were located terminally on the long arms of two chromosome pairs withvariations in the NOR-bearing chromosome. The techniques of karyotypeidentification, banding, and NORs employed in this study may serve asuseful indexes in future research on cytogenetics in Haliotidae.[References: 30] 收起