摘要 : Cells of suspension culture Citrullus vulgaris cv. "Samara" were permeabilized by Tween 80 and immobolized by gluttaraldehlyde. The highest melibiase activity was at pH 5.4 and 60 degrees C. The hydrolasis of substrate was linear ... 展开 Cells of suspension culture Citrullus vulgaris cv. "Samara" were permeabilized by Tween 80 and immobolized by gluttaraldehlyde. The highest melibiase activity was at pH 5.4 and 60 degrees C. The hydrolasis of substrate was linear for 3.5 h, reaching 65-70% conversion of the substrate. The cells, characterized by high enzyme activity and stability, in long-term storage, showed convenient physical-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts). 收起