摘要 : Plasma membranes isolated from cells of the halophilic purple nonsulfur bacterium Rhodospirillum salinarum grown in light or in the dark were examined. Membranes isolated from cells grown aerobically in the dark contained three b-... 展开 Plasma membranes isolated from cells of the halophilic purple nonsulfur bacterium Rhodospirillum salinarum grown in light or in the dark were examined. Membranes isolated from cells grown aerobically in the dark contained three b-type and two c-type membrane-bound cytochromes with E-m.7 of +180, +72 and -5 mV (561-575 nm), and +244 and +27 mV (551-540 nm), respectively. Conversely, membranes isolated from cells grown anaerobically in the light contained two 6-type and five c-type haems with E-m.7 of +60 and -45 mV and +390, +250, +135, -20 and -105 mV, respectively, In addition to haems of the b- and c-type, two haems of the a-type (E-m.7 of +325 and +175 mV) were present only in cells grown in the dark. Four soluble cytochromes of the c type, but not cytochrome c(2), along with two high-potential iron-sulfur proteins (HiPIP iso-1 and iso-2) were also identified in cells grown aerobically. Inhibitory studies showed that 85-90% of the respiratory activity was blocked by very low concentrations of cyanide, antimycin A and myxothiazol (50, 0.1 and 0.2 mM, respectively). These results taken together were interpreted to show that the oxidative electron transport chain of Rsp. salinarum urn is linear, leading to a membrane-bound oxidase of the aa(3) type in cells grown in the dark, while no significant cytochrome oxidase activity is catalyzed by photosynthetic membranes, These features suggest that this halophilic species is unique among the genus Rhodospirillum and that it also differs from other facultative phototrophs (e.g., Rhodobacter species) in that it does not contain either cytochrome c(2) or a branched respiratory chain. 收起