摘要 : Lanthanide fluorescence enhancement on complexation with calf thymus DNA was studied in aqueous solution. The DNA sensitized and enhanced fluorescence of terbium and europium by nearly two orders of magnitude. By applying this lig... 展开 Lanthanide fluorescence enhancement on complexation with calf thymus DNA was studied in aqueous solution. The DNA sensitized and enhanced fluorescence of terbium and europium by nearly two orders of magnitude. By applying this ligand sensitized lanthanide fluorescence enhancement, DNA could be estimated at 10 ppb level. Further, effect of addition of TOPO in Triton X-100 micellar medium to Tb-DNA complex in solution was also studied. On addition of TOPO, no synergistic terbium fluorescence enhancement was observed. (C) 2002 Elsevier Science B.V. All rights reserved. [References: 21] 收起