摘要 : This paper provides an overview of The Honey Bee Genome Sequencing Project (HBGSP), from its conception from 1998 to 2001, to the challenges it faced since it began in 2002, the complications it encountered (e.g. under-representat... 展开 This paper provides an overview of The Honey Bee Genome Sequencing Project (HBGSP), from its conception from 1998 to 2001, to the challenges it faced since it began in 2002, the complications it encountered (e.g. under-representation of AT-rich regions of the genome among the small insert shotgun libraries constructed in Escherichia coli for the bulk of the sequencing; and the lack of BAC clones), the corresponding solutions developed, and the achievements attained to date. In addition to the draft assembly (i.e. a consensus sequence good enough to represent nearly all genes at a quality sufficient for use in searches), a collection of single nucleotide polymorphisms produced as part of the project is also briefly discussed. The HBGSP has so far produced a prodigious amount of information, and online resources and database development is proceeding aggressively to manage this.. 收起