摘要 : The present work aimed to evaluate the influence of surface chemistry of silver nanoparticles on the reprotoxicity in mice model. The toxicity of citrate-reduced nanoparticles has been challenged using plant material epicatechin, ... 展开 The present work aimed to evaluate the influence of surface chemistry of silver nanoparticles on the reprotoxicity in mice model. The toxicity of citrate-reduced nanoparticles has been challenged using plant material epicatechin, a bio-flavonoid, that has known protective roles in the living system. The identity of the particles was confirmed using UV-Vis spectroscopy, DLS and zeta potential, HR-SEM, and EDAX. Following characterization, both citrate-reduced and epicatechin-coated particles were separately injected intraperitoneally on 0, 3rd, 7th, 14th, 21st, and 28th day. On the 35th day, mice were operated for the measurement of various reproductive indices. It was found that both these particles had toxic effect at variable dosages ranging from 1 to 5 mg/kg of body weight on male reproductive tissue. But significant differences at the level of toxicity were indicated by the parameters of reproductive indices like histology, spermatozoa parameters, serum testosterone level, level of sialic acid and fructose in epididymis and seminal vesicle, serum catalase, and reactive oxygen species production in cauda epididymal spermatozoa. Epicatechin-protected silver nanoparticles showed significant reduction in toxicity at all parameters at higher dose level. The hypo-osmotic swelling test and eosin-nigrosin staining indicated more protection against oxidative damage in EcAgNP-exposed spermatozoa than the CAgNPs. The different surface chemistry imposed by epicatechin certainly provided beneficial roles with anti-oxidant property against repro-nanotoxic effect imposed by the surface of chemically reduced particles in male mice reproductive system. 收起