摘要
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Bacteria of the genus <i>Bacillus</i> are well known to possess antagonistic activity against numerous plant pathogens. In the present study, 11 strains of <i>Bacillus</i> spp. were isolated from a brackish environment and assayed...
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Bacteria of the genus <i>Bacillus</i> are well known to possess antagonistic activity against numerous plant pathogens. In the present study, 11 strains of <i>Bacillus</i> spp. were isolated from a brackish environment and assayed for biocontrol activity under <i>in vitro</i> and <i>in vivo</i> conditions. Among the 11 isolates tested, nine isolates effectively inhibited the growth of various plant pathogens, namely <i>Phytophthora capsici</i>, <i>Phytophthora citrophthora</i>, <i>Phytophthora citricola</i>, <i>Phytophthora sojae</i>, <i>Colletotrichum coccodes</i>, <i>Colletotrichum gloeosporioides</i>, <i>Colletotrichum acutatum</i>, <i>Rhizoctonia solani</i>, <i>Fusarium solani</i>, <i>Fusarium graminearum</i>, <i>Pyricularia</i> spp., and <i>Monilina</i> spp. The effective isolates were further screened for suppression of <i>Phytophthora</i> blight of pepper plants under greenhouse conditions. The isolate SB10 exhibited the maximum (72.2%) ability to reduce the disease incidence and increased (32.2%) the vigour index of <i>Capsicum annuum</i> L. plants. Antifungal compounds produced by isolate SB10 were highly thermostable (100 degrees C for 30 min). Matrix-Assisted Laser Desorption Ionization-Time of Flight mass spectrometry of the antifungal compounds revealed three lipopeptide complexes, namely the surfactins, the iturins, and the fengycins, which are well-known antifungal compounds produced by <i>Bacillus</i> spp.
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