摘要 : One hundred and fourteen kilobnse pairs (kb) of contiguous genomic sequence have been determined immediately distal to the hiss genetic marker located about 0.9 Mb from the centromere on the long arm of Schizosaccharomyces pombe c... 展开 One hundred and fourteen kilobnse pairs (kb) of contiguous genomic sequence have been determined immediately distal to the hiss genetic marker located about 0.9 Mb from the centromere on the long arm of Schizosaccharomyces pombe chromosome 2. The sequence is contained in overlapping cosmid clones c16H5, c12D12, e24C6 and c19G7, of which 20 kb are identical to previously reported sequence from clone c21H7. The remaining 93 781 bp of sequence contains 10 known genes (cdcl4, cdmI, cpsl, gpal, msh2, pck2, ripl, rps30-2, sadl and ubll), 32 open reading frames (ORFs)capable of coding for proteins of at least 100 amino acid residues in length, one 5S rRNA gene, one tRNA~(Pro) gene, one lone Tfl-type long terminal repeat (LTR) and one lone Tf2-type LTR. There is a density of one protein-coding gene per 2.2 kb and 22 of the 42 ORFs (52%) incorporate one or more introns. Twenty-one of the novel ORFs show sequence similarities which suggest functions of their products, including a cyclin C, a MADS box transcription factor, mad2-1ike protein, telomere binding protein, topoisomerase II-associated protein, ATP-dependent DEAH box RNA helicase, Gl0 protein, ubiquitin-activating el-like enzyme, nucleoporin, prolyl-tRNA synthetase, peptidylprolyl isomerase, #delta#-1-pyrroline-5- carboxylate dehydrogenase, protein transport protein, coatomer epsilon, TCP-I chaperouin, #beta#-subunit of 6-phosphofructokinase, aminodeoxychorismate lyase, a phosphate transport protein and a thioredoxin. The sequence has been submitted to the EMBL database under Accession Nos AL021839, AL022104, AL031786 and AL035085. 收起