摘要 : Interspecific hybrids of mung bean (Vigna radiata) with cowpea (V. unguiculata) were generated in an otherwise incompatible cross after overcoming crossability barriers using in vitro culture techniques. Different pre- and post-fe... 展开 Interspecific hybrids of mung bean (Vigna radiata) with cowpea (V. unguiculata) were generated in an otherwise incompatible cross after overcoming crossability barriers using in vitro culture techniques. Different pre- and post-fertilization barriers were studied and overcome by the application of hormones. Gibberellic acid (GA) application to the pollinated flower buds was more effective in increasing pollen germination (44%) and pollen tube growth (31%). The inhibition of pollen tube growth in the styles of the crosses was reduced by 15-20% after GA application as compared to their selfed parents. Pod set, pod harvest and flower and pod retention percentages were higher in the kharif (July to October) season as compared to the spring (March to June) season. Low temperatures of 26 to 30deg C with 75 to 85% humidity in the kharif season were more favourable for overcoming crossability barriers than the high temperatures (32 to 36deg C) and low humidity (40 to 50%) of the spring season. GA treatment significantly increased the pod harvest in the crosses, and hybrid pod growth could be sustained for 9-10 days making it possible to establish embryo cultures. Some 10% of the total embryos cultured resulted in successful plantlet formation on MS medium supplemented with 0.4 mg NAA and 1 mg benzyladenine/litre. 收起