摘要 : Musa basjoo (MB) is a species of the banana plant belonging to the genus Musa that has been used as a folk medicine. However, evidence-based biological activities and the molecular mechanism of action of MB are unknown. Thus, the ... 展开 Musa basjoo (MB) is a species of the banana plant belonging to the genus Musa that has been used as a folk medicine. However, evidence-based biological activities and the molecular mechanism of action of MB are unknown. Thus, the aim of the present study was to examine whether the crude dried leaf extracts of MB inhibit the growth of colorectal (HT29 and HCT116) and other types (HepG2, MCF-7 and PC-3) of human cancer cell lines. Crude extracts of MB inhibited the growth of cells with IC50 values of 136 μg/ml (acetone extract, HT29), 51 μg/ml (acetone extract, HCT116), 45 μg/ml (acetone extract, HepG2), 40 μ/g ml (acetone extract, MCF-7), 29 μg ml (acetone extract, PC-3), 175 μg/ml (methanol extract, HT29), 137 μg/ml (methanol extract, HCT116), 102 μg/ml (methanol extract, HepG2), 85 μg/ml (methanol extract, MCF-7), and 85 μg/ml (methanol extract, PC-3) in colony formation assays, and 126 μg/ml (acetone extract, HT29), 68 μg/ml (acetone extract, HCT116), 260 μg/ml (methanol extract, HT29), and 216 μg/ml (methanol extract, HCT116) in MTT assays. Thin layer chromatography analysis revealed the potential existence of aromatic compounds in the acetone extract of MB. Flow cytometric analysis indicated that the percentage of cells in G1 increased, and this was associated with a concomitant decrease of cells in the S and/or G2-M phases of the cell cycle. When colorectal cancer cells were treated with acetone extract of MB, there was a marked decrease in the levels of expression of the cyclin D1, cyclin E, cdk2 and cdk4 proteins and a marked increase in the levels of the expression of the p21CIP1, p27KIP1, and p53 proteins, but those of apoptosis-associated protein PARP did not change. There was a tendency for acetone extract of MB to inhibit xenograft tumor growth in mice. Collectively, the crude extracts of MB contain active components that exert growth inhibition of human cancer cells. This is the first systematic study of the anticancer activity of MB and may broaden insights into the possible clinical approach of specific herbal medicines. 收起