摘要: Alpha-2U globulin is a low molecular weight urinary protein which may be associated with a hydrocarbon-induced proximal tubular cell degeneration in the male rat kidney. A new method was developed to obtain monospecific immunologi... 展开 Alpha-2U globulin is a low molecular weight urinary protein which may be associated with a hydrocarbon-induced proximal tubular cell degeneration in the male rat kidney. A new method was developed to obtain monospecific immunologic reagents for alpha-2U globulin using diafiltration, anion-exchange and hydroxylapatite chromatography. Isoelectric focusing techniques were developed to isolate the major isoelectric variants of the alpha-2U globulin molecule and to assess changes in alpha-2U globulin after experimental exposure to hydrocarbon compounds. Alpha-2U globulin was isolated from the urine of albino and pigmented male rats to study strain susceptibility to the nephrotoxic process. An alpha-2u globulin isoelectric variant profile distinguishing albino from non-albino male rats was not apparent, however, strain differences were revealed. Fischer 344 male rats appear to have higher levels of the isoelectric variants than the other strains studied. These findings suggest that if a strain susceptibility to the hydrocarbon-induced nephrotoxic lesion exists, it may be associated with the alpha-2U globulin isoelectric variant profile. 收起