摘要 : Abstract For the first time, five lead-resistant bacteria were isolated from the marine water and sediments of Versova Creek, Mumbai, India, and analysed for their bioflocculation-assisted bioremediation potential. Based on 16 S r... 展开 Abstract For the first time, five lead-resistant bacteria were isolated from the marine water and sediments of Versova Creek, Mumbai, India, and analysed for their bioflocculation-assisted bioremediation potential. Based on 16 S rRNA sequencing and BLAST, the isolates matched the genera Bacillus and Enterobacter. They exhibited robust growth in media amended with 1600 mg/l of lead nitrate, with a minimum inhibitory concentration (MIC) of 2000 mg/l. The optimum growth parameters were determined to be 37ºC and pH 7. Gram staining and colony morphology identified PbR01 and PbR02 as Gram-positive, while PbR03, PbR04, and PbR05 were Gram-negative. Bacillus sp. AKVPbR02 demonstrated exceptional lead removal efficiency, eliminating 74% and 89.5% of Pb(II) at an initial concentration of 400 mg/l after 72 and 120 h of incubation, respectively. Enterobacter sp. AKVPbR04 also exhibited high removal efficiency, achieving 89.49% removal by the 5th day (120 h) of incubation. Other strains, including Enterobacter sp. AKVPbR03, Bacillus sp. AKVPbR01, and Enterobacter sp. AKVPbR05, removed 87.70%, 83.52%, and 82.76% of Pb(II), respectively, by the same incubation period. The isolates displayed diverse biochemical profiles. All demonstrated urease activity except PbR05, while only PbR02 exhibited β-glucuronidase activity. Most isolates could metabolize a range of sugars, including sucrose, D-sorbitol, and D-trehalose. Nitrogen source utilization varied, with all isolates capable of utilizing lysine and ornithine except PbR05. Antibiotic sensitivity testing revealed varied resistance and susceptibility patterns. For example, PbR01, PbR02, PbR04, and PbR05 were sensitive to azithromycin and erythromycin, while PbR03 exhibited resistance. Most isolates showed resistance to penicillin and piperacillin but were sensitive to ampicillin/sulbactam.Graphical Abstract 收起