摘要 : UV-visible spectra of three solvatochromic dyes, 4,6-dichloro-2-[2-(1-methyl-4-pyridinio)vinyl]phenolate, N-methyl-2-nitroaniline, and 2-nitroaniline, are employed to characterize the octadecyl-derivatized silica (ODS2) stationary... 展开 UV-visible spectra of three solvatochromic dyes, 4,6-dichloro-2-[2-(1-methyl-4-pyridinio)vinyl]phenolate, N-methyl-2-nitroaniline, and 2-nitroaniline, are employed to characterize the octadecyl-derivatized silica (ODS2) stationary phase and the corresponding mobile phases used in reversed-phase liquid chromatography. The mobile phases studied are methanol-water, acetonitrile-water, and tetrahydrofuran-water, with composition ranges from 100% to 5% of organic modifier. Across the whole mobile phase composition range, the ODS2 stationary phase shows three distinct behaviors upon variation of the mobile phase composition, which are described as non-retentive, solvated, and collapsed. 收起