摘要 : Epoxyeicosatrienoic acids (EETs) are cytochrome P450 metabolites of arachidonic acid involved in the regulation of vascular tone. Despite the importance of EETs in a variety of physiological effects, few methods have been develope... 展开 Epoxyeicosatrienoic acids (EETs) are cytochrome P450 metabolites of arachidonic acid involved in the regulation of vascular tone. Despite the importance of EETs in a variety of physiological effects, few methods have been developed to quantity them in human blood. This led us to develop a method by GC/MS with negative ion chemical ionization. As EETs are primarily located in phospholipids, red blood cells (RBCs) and plasma phospholipids were hydrolyzed with phospholipase A(2) after a solid phase extraction. Then, EETs were derivatized as pentafluorobenzyl esters, and [H-2(8)]-arachidonic acid was used as internal standard for quantification. EETs were found to be at concentrations of 106 +/- 37 ng mL(-1) in plasma and 33.4 +/- 8.5 ng/10(9) RBCs (mean +/- S.D.) in 10 healthy volunteers. Their amount in RBCs was 3-fold that in plasma: both parameters proved to be well correlated. (C) 2008 Elsevier B.V. All rights reserved. 收起