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Tan spot, caused by the fungus Pyrenophora tritici-repentis (Died.) Drechs is an important foliar disease of wheat (Triticum aestivum L.). From a set of phenotypically and molecularly characterized set of Argentinean isolates, two...
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Tan spot, caused by the fungus Pyrenophora tritici-repentis (Died.) Drechs is an important foliar disease of wheat (Triticum aestivum L.). From a set of phenotypically and molecularly characterized set of Argentinean isolates, two isolates H0019 and H0120 which do not correspond to known races of the pathogen were selected. Segregation for resistance among a set of recombinant inbred lines bred from the cross 'W7984' x cv. 'Opata 85' was used to identify the basis for resistance at the seedling stage, against those fungal isolates (H0019 and H0120), across three independent environments. On the basis of the mean performance across all three environments, a QTL against chlorosis located on the 6AS and linked to the RFLP locus Xksuh4c was significant for both isolates (with a LOD of 3.76 for isolate H0019 and 5.87 for H0120).
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Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these population...
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Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these populations. In this study, quantitative and qualitative traits along with molecular markers were used to evaluate 14 populations from the Instituto Nacional de Tecnologia Agropecuaria (INTA) sunflower germplasm collection. These populations were chosen to represent historically important accessions that still play a central role within the INTA sunflower breeding programme due to their extensive variation in diverse agronomically important traits. Nine quantitative and eight qualitative agro-morphological descriptors were recorded and compared with those of a larger set of accessions representative of the phenotypic diversity of the sunflower collection. Molecular characterization was conducted on a total of 311 individuals using 16 microsatellite markers. Overall, the average gene diversity was 0.56 and the average number of alleles per locus was 6.25. No statistically significant differences in genetic diversity were detected between the OPs and CPs. Global estimates of FST revealed very high levels of differentiation among accessions (FST=0.413, P<0.05). Population structure analyses were consistent with the observed levels of differentiation and identified two major groups. The results of this work show that high global diversity is preserved within the accessions analysed here. Additionally, this study provides a set of reliable and discriminant markers for the cost-effective molecular characterization of sunflower accessions, along with the guidelines for the delineation of sampling strategies for OPs and CPs, thus aiding the efficient management and exploitation of sunflower germplasm collections.Digital Object Identifier http://dx.doi.org/10.1017/S1479262113000075
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Fungi of yield soils represent a significant portion of the microbial biomass and reflect sensitivity to changes in the ecosystem. Our hypothesis was that crops included in cropping regimes under the zero tillage system modify the...
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Fungi of yield soils represent a significant portion of the microbial biomass and reflect sensitivity to changes in the ecosystem. Our hypothesis was that crops included in cropping regimes under the zero tillage system modify the structure of the soil fungi community. Conventional and molecular techniques provide complementary information for the analysis of diversity of fungal species and successful information to accept our hypothesis. The composition of the fungal community varied according to different crops included in the cropping regimes. However, we detected other factors as sources of variation among them, season and sampling depth. The mixed cropping regimes including perennial pastures and one crop per year promote fungal diversity and species with potential benefit to soil and crop. The winter season and 0-5 cm depth gave the largest evenness and fungal diversity. Trichoderma aureoviride and Rhizopus stolonifer could be used for monitoring changes in soil under zero tillage.
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The concept of fire regime refers to a variety of fire characteristics occurring at a given place and period of time. Understanding fire regimes is relevant to fire ecology and fire management because it provides a better understa...
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The concept of fire regime refers to a variety of fire characteristics occurring at a given place and period of time. Understanding fire regimes is relevant to fire ecology and fire management because it provides a better understanding of effects of fire as well as the potential effects of different future scenarios. Recent changes in the traditional fire regimes linked to climate and socioeconomic transformations in European Mediterranean areas have influenced fire regimes and their effects on both ecosystems and people. This paper presents a methodology for characterising fire regimes based on historical fire statistics. The analysis includes three dimensions: density, seasonality and interannual variability. The raw records were pre-processed to eliminate errors, and a principal component analysis was performed to identify the primary factors involved in the variation. A cluster analysis was then used to define the fire regimes. Approximately 38% of the spatial cells examined were found to have significant fire activity, but in spite that fires are important in these areas, fire activity showed a high interannual variability. Four fire regimes in the Spanish peninsular territory were described in terms of the density and seasonality of fire activity.Digital Object Identifier http://dx.doi.org/10.1071/WF12061
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The presence of Fusarium species in cultivated soils is commonly associated with plant debris and plant roots. Fusarium species are also soil saprophytes. The aim of this study was to examine the occurrence and distribution of soi...
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The presence of Fusarium species in cultivated soils is commonly associated with plant debris and plant roots. Fusarium species are also soil saprophytes. The aim of this study was to examine the occurrence and distribution of soil Fusarium spp. at different soil depths in a zero tillage system after the wheat was harvested. Soil samples were obtained at three depths (0-5 cm, 5-10 cm and 10-20 cm) from five crop rotations: I, conservationist agriculture (wheatsorghum-soybean); II, mixed agriculture/livestock with pastures, without using winter or summer forages (wheatsorghum-soybean-canola-pastures); III, winter agriculture in depth limited soils (wheat-canola-barley-late soybean); IV, mixed with annual forage (wheat-oat/Vicia-sunflower); V, intensive agriculture (wheat-barley-canola, with alternation of soybean or late soybean). One hundred twenty two isolates of Fusarium were obtained and identified as F. equiseti, F. merismoides, F. oxysporum, F. scirpi and F. solani. The most prevalent species was F. oxysporum, which was observed in all sequences and depths. The Tukey's test showed that the relative frequency of F. oxysporum under intensive agricultural management was higher than in mixed traditional ones. The first 5 cm of soil showed statistically significant differences (p=0.05) with respect to 5-10 cm and 10-20 cm depths. The ANOVA test for the relative frequency of the other species as F. equiseti, F. merismoides, F. scirpi and F. solani, did not show statistically significant differences (p收起
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Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against Pyrenophora tritici-repentis (Died.) Drechsler (anamorph Drechslera tritici-repentis) (Di...
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Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for their potential as biocontrol agents against Pyrenophora tritici-repentis (Died.) Drechsler (anamorph Drechslera tritici-repentis) (Died.) Shoem (Dtr), the causal agent of tan spot of wheat. Endophytes were screened using dual culture techniques and examining the effect on growth, sporulation and the antifungal activity in greenhouse assays. The most of endophytes tested significantly reduced Dtr growth compared to the control except Rhodotorula rubra from 11 to 15 days post inoculation. Trichoderma hamatum, Penicillium sp., Bacillus sp. and Paecilomyces lilacinus significantly reduced the colony diameter of the pathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or the mycelia of D. tritici-repentis. In addition, two endophytes, Bacillus sp. and Fusarium sp., reduced significantly the percent spore germination of Dtr compared to the control by 82% and 52% respectively. In greenhouse experiment T. hamatum, Chaetomium globosum and Fusarium sp. significantly (P <= 0.05) reduced the average disease severity on all three leaves compared to the control. However, the best antagonistic effect was shown with T. hamatum as it resulted in the greatest suppression in the greenhouse and in the dual-plate assays. Likewise, Bacillus sp. was other highlighted microorganism that antagonized the pathogen in in vitro assays. From our promising results, we conclude that endophytes have potential in the biological control of tan spot of wheat caused by D. tritici-repentis, particularly T. hamatum and Bacillus sp. (C) 2015 Elsevier Inc. All rights reserved.
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Fusarium head blight is a fungal disease caused by a complex of Fusarium species on cereals, such as barley and wheat. It has economic impacts due to yield reductions and mycotoxin contamination. As barley production has increased...
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Fusarium head blight is a fungal disease caused by a complex of Fusarium species on cereals, such as barley and wheat. It has economic impacts due to yield reductions and mycotoxin contamination. As barley production has increased considerably in the last 5 years in Argentina, a survey was conducted for identifying Fusarium species associated with barley grains. Fusarium cerealis was isolated and identified based on morphological and molecular analysis. The potential production of nivalenol and zearalenone was assessed using specific PCR assays. Koch's postulates were carried out to confirm the pathogenicity of the fungus.
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In December 2009, fungal species in wheat seeds from a field in Azul, Buenos Aires, Argentina, were studied. Eight isolates morphologically similar to Fusarium species were observed after 6 days of incubation. For identification, ...
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In December 2009, fungal species in wheat seeds from a field in Azul, Buenos Aires, Argentina, were studied. Eight isolates morphologically similar to Fusarium species were observed after 6 days of incubation. For identification, monosporic isolates were transferred onto potato dextrose agar (PDA) and carnation leaf agar (CLA). One isolate, when grown on PDA, rapidly produced abundant, dense, white, aerial mycelium that became pink with age and formed red pigments in the medium. On CLA, macroconidia were abundant, relatively slender, curved to lunate, and 3-5 septate. Microconidia were abundant, napiform, oval or pyriform, 0-1 septate, and commonly clustered in false heads. Chlamydospores were absent. The fungus was identified as F. tricinctum [Gibberella tricincta] based on morphology. The genomic DNA of the isolate was extracted and the internal transcribed spacer (ITS) and the translation elongation factor (TEF) regions were PCR-amplified using primer pairs ITS3/ITS4 (4) and EF-1/EF-2 (2), respectively. The ITS sequence (accession no. HM635739) showed 100% similarity with several F. tricinctum sequences (accession nos. HM068317, FN598932 and EF589873) but also with other Fusarium species, such as F. acuminatum. The TEF sequence (accession no. HQ214681) showed 99-100% similarity with accession nos. HM068307, EU744838 and EU744837 of F. tricinctum. This is thought to be the first report of F. tricinctum on wheat in Argentina.Digital Object Identifier http://dx.doi.org/10.1094/PDIS-07-10-0485
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