摘要 :
Insulin-independent actions of glucagon-like peptide-1 (GLP-1) are not yet clear in ruminants. Four Suffolk mature wethers (60.0 +/- 6.7kg body weight (BW)) were intravenously infused with insulin (0.5mU/kg BW/min; from 0 to 90min...
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Insulin-independent actions of glucagon-like peptide-1 (GLP-1) are not yet clear in ruminants. Four Suffolk mature wethers (60.0 +/- 6.7kg body weight (BW)) were intravenously infused with insulin (0.5mU/kg BW/min; from 0 to 90min) and GLP-1 (0.5g/kg BW/min; from 60 to 150min) with both hormones co-administered from 60 to 90min, in a repeated-measure design under euglycemic clamp for 150min, to investigate whether GLP-1 has insulin-independent actions. Jugular blood samples were taken at 15-min intervals for plasma hormones and metabolites analysis. Compared to baseline concentrations (at 0min), insulin infusion decreased (P<0.05) plasma concentrations of glucagon, non-esterified fatty acids (NEFA), lactate, nonessential amino acids (NEAA), branched-chain amino acids (BCAA), total amino acids (TAA) and urea nitrogen (UN). Insulin plus GLP-1 infusion induced a greater increase (P<0.05) in plasma concentrations of insulin and triglyceride (TG), but decreased (P<0.05) glucagon, total cholesterol (T-Cho), NEAA and UN plasma concentrations. GLP-1 infusion increased (P<0.05) NEFA, -hydroxybutyrate and TG, but decreased (P<0.05) glucagon, T-Cho, NEAA, BCAA and UN plasma concentrations. In conclusion, GLP-1 exerts extrapancreatic roles in ruminants not only insulin-independent but probably, in contrast to non-ruminants, antagonistic to insulin effects.
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