将单因素实验结果与响应面法相结合,对高产Monacolin K的紫色红曲霉Mp-24菌株进行发酵工艺条件优化。通过摇瓶发酵对碳源、氮源、碳源含量、氮源含量、培养时间等进行单因素优化,确定Mp-24菌株摇瓶发酵适宜条件：乳糖为碳源、酵母膏为氮源、碳源含量7%、氮源含量2%、培养时间12 d,Monacolin K产量为167 mg/L。应用Box-Behnken中心组合试验设计建立数学模型,进行响应面分析优化发酵条件,结果显示最佳发酵工艺条件为：碳源(乳糖)8%,氮源(酵母膏)3%,培养时间11 d,在此条件下Monacolin K的含量达到247.8 mg/L,比优化前提高1.5倍。
Response surface methodology and single factor experiment were combined to optimize the submerged culture conditions for the production of Monacolin K using Monascus purpureus strain Mp-24 . Through analyzing the influence of carbon sources, nitrogen sources, carbon content, nitrogen content and culture time, the results showed that optimal fermentation conditions were as follows:lactose( carbon source) , yeast extract( nitrogen source) , carbon content of 7%, nitrogen content of 2% and culture time of 12 d. Monacolin K yield was 167 mg/L. After using Box-Behnken central composite design and response surface analysis, the optimum conditions were: carbon ( lactose) of 8%, nitrogen ( yeast extract) of 3% and culture time of 11 d. Under the optimized conditions, the content of Monacolin K reached 247. 8 mg/L. The optimized fermentation conditions resulted in 1 . 5 times increase of Monacolin K yield in comparison with that of the original conditions.
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