姜黄素改善大鼠创伤性脑损伤炎性反应及预后OA

Objective To investigate the therapeutic effect and the potential mechanism of curcumin for treating trau-matic brain injury(TBI). Methods 12 SD rats were randomly divided into the curcumin group(6 cases) and the DMSO group (control,6 cases). After TBI model was established,each rat in the curcumin group was treated with curcumin for 3 d,while the DMSO group was given the equal quantity of DMSO by intraperitoneal injection. The body mass and neurological functional score were daily measured from the beginning to the end of experiment. On 5 d(peak period),the brain tissue was taken for observing the macrophage/microglia inflammatory infiltration in the brain by the immunohistochemistry staining. The real time quantitative RT-PCR was performed for detecting the relative expression of TNF-αand IL-1β. On 25 d(recovery period),the brain tissue was taken for detecting the relative expressions of IL-10 and TGF-βin brain by RT-PCR. Results The neurological functional scores showed that the severity degrees of the rat TBI were significantly decreased after curcumin treatment ,indicating that curcumin could inhibit the macrophage infiltration and microglia activation in the rat brain tissue ,inhibited the expression of TNF-αand IL-1βin the brain tissue during the peak period and promoted the expression of IL-10 and TGF-βduring the recovery period of TBI. Conclusion Curcumin has the curative effect on TBI,which may be related with curcumin inhibiting the inflammatory reaction after TBI and promoting the tissue recovery.